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antitnfα  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antitnfα
    Antitnfα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antitnf%CE%B1/TNF-alpha+Rabbit+mAb/pm41239371-69-42-44
    Average 95 stars, based on 166 article reviews
    antitnfα - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: A glucose time in range of 70% attenuates the senescence-inducing and pro-inflammatory effects of hyperglycemia.
    Article Snippet: For Western blots, 50 μg of lysate was separated by electrophoresis using PAGE gels (NP0335BOX, NuPAGETM Bis–Tris Mini Protein Gels, 4–12%, Thermo Fisher) and transferred to nitrocellulose membranes (GE10600020, 0.45 μm, Merck). .. After blocking with 5% non-fat dried milk (70,166-500G, Merck) or 5% bovine serum albumin (A1470-100G, Merck), membranes were incubated overnight at 4 °C with the following primary antibodies: anti-IL-1β (ab254360, Abcam), anti-IL-6 (#12,153; Cell Signalling), anti-IL-8 (ab110727; Abcam), anti-CXCL1 (#24,376; Cell Signalling), antiTNFα (#6945; Cell Signalling), anti-PAI-1 (#11,907; Cell Signalling), and anti-Vinculin (#13,901; Cell Signalling) all diluted 1:1000, Secondary IgG HP-conjugated anti-rabbit HRP-linked antibody (#7074; Cell Signalling, 1:3000) were applied for 1 h at room temperature. .. Immunoreactive proteins were revealed with WESTAR ETA ULTRA C 2.0 (XLS075,0100, Cyanagen) using UVITEC Alliance Q9.

    Incubation:

    Article Title: A glucose time in range of 70% attenuates the senescence-inducing and pro-inflammatory effects of hyperglycemia.
    Article Snippet: For Western blots, 50 μg of lysate was separated by electrophoresis using PAGE gels (NP0335BOX, NuPAGETM Bis–Tris Mini Protein Gels, 4–12%, Thermo Fisher) and transferred to nitrocellulose membranes (GE10600020, 0.45 μm, Merck). .. After blocking with 5% non-fat dried milk (70,166-500G, Merck) or 5% bovine serum albumin (A1470-100G, Merck), membranes were incubated overnight at 4 °C with the following primary antibodies: anti-IL-1β (ab254360, Abcam), anti-IL-6 (#12,153; Cell Signalling), anti-IL-8 (ab110727; Abcam), anti-CXCL1 (#24,376; Cell Signalling), antiTNFα (#6945; Cell Signalling), anti-PAI-1 (#11,907; Cell Signalling), and anti-Vinculin (#13,901; Cell Signalling) all diluted 1:1000, Secondary IgG HP-conjugated anti-rabbit HRP-linked antibody (#7074; Cell Signalling, 1:3000) were applied for 1 h at room temperature. .. Immunoreactive proteins were revealed with WESTAR ETA ULTRA C 2.0 (XLS075,0100, Cyanagen) using UVITEC Alliance Q9.

    Western Blot:

    Article Title: Loss of Ca 2+ entry via Orai-TRPC1 induces ER stress, initiating immune activation in macrophages.
    Article Snippet: .. The following primary antibodies were used for western blot analysis (all at 1:1000): anti-Orai1 (Thermo Fisher, PA5-26378), anti-TRPC1 (Alamone, ACC-010), anti-STIM1 (D88E10), anti-CHOP (L63F7), anti-PDI (C81H6), anti-GRP94 (D6X2Q), anti-NFκB p65 (p65) (8242S), anti-pNFκB p65 (pp65) (3033S), anti-Bax (D2E11), anti-IREI1α (14C10), anti-Bcl2 (D17C4), anti-ATF6 (D4Z8V), anti-PERK (C33E10), anti-pPERK (16F8), antiTNFα (D2D4), anti-IL-6 (D5W4V), anti-IL-1β (D6D6T), anti-HMGB1 (D3E5), anti-Histone 3 (D1H2), anti-HSP70 (4872S), anti-Rb (D20), anti-pRB (D20B12), anti-Cyclin D1 (92G2), anti-p53 (1C12), anti-pp53 (Ser15), anti-LC3B (D11), anti-caspase 3 (9662S), anti-caspase 9 (C9) and anti-β-actin (4970S) (all from Cell Signaling Technology). .. HRP-conjugated goat anti-rabbit IgG (7074S) and goat anti-mouse IgG (7076S) (both from Cell Signaling Technology) were used as secondary antibodies.



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